2× SYBR cagaaran qPCR MIX (oo leh ROX sare)

Tilmaamaha alaabta

Alaabtan, 2×SYBR cagaaran qPCR MIX, waxay ku timaadaa hal tubo oo ka kooban dhammaan qaybaha looga baahan yahay kordhinta PCR iyo ogaanshaha, oo ay ku jiraan Taq DNA poLymerase, SYBR green I dye, High ROX Reference Dye, dNTPs, Mg2+, iyo PCR baffer.
SYBR cagaarka I dheeha waa midab cagaaran oo fluorescent ah kaas oo ku xidhan DNA-da laba-geesoodka ah (DNA, dsDNA) double helix small groove region.SYBR cagaaran I fluoresces daciif ah ee gobolka xorta ah, laakiin mar ay ku xidho DNA laba-xarun ah, fluorescence si weyn ayaa loo wanaajiyey. Tani waxay suurtogal ka dhigaysaa in la qiyaaso qadarka DNA-da laba-geesoodka ah ee la soo saaro inta lagu jiro kordhinta PCR iyadoo la ogaanayo xoojinta fluorescence.
ROX waxa loo isticmaalaa dheeha sixitaanka si loo saxo isbedbedelka fluorescence ee aan xidhiidh la lahayn PCR, sidaas awgeed waxa la yareynayaa kala duwanaanshaha saxafada. Kala duwanaanshiyaha noocan oo kale ah waxaa sababi kara arrimo kala duwan, sida qaladka pipette ama saamiga uumi-baxa. Qalab qiyaaseedka fluorescence kala duwan ayaa leh baahiyo kala duwan oo loogu talagalay ROX, badeecadanna waxay ku habboon tahay falanqeeyayaasha qiyaasaha fluorescence ee u baahan sixitaanka Sare ee ROX.