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Qalabka Nadiifinta DNA-da ee MagaPure Oryza sativa L.
Hordhac kooban
Qalabkani wuxuu qaataa nidaam gaar ah oo gaar ah oo la horumariyey oo la hagaajiyey iyo kuul birlab ah oo si gaar ah ugu xidhma DNA-da, kaas oo si dhakhso ah u xidhi kara, u nuugi kara, u kala saari kara oo u nadiifin kara asiidhyada nukliyeerka, iyadoo si wax ku ool ah uga saari kara wasakhda sida polysaccharides iyo isku-dhafka polyphenol ee dhirta. Aad bay ugu habboon tahay in laga soo saaro DNA-da genomic unugyada caleenta dhirta. Iyada oo la taageerayo isticmaalka Bigfish Magnetic Bead Nucleic Acid Extractor, aad bay ugu habboon tahay soo saarista otomaatiga ah ee cabbirrada muunadaha waaweyn. DNA-da genomic-ga ee la soo saaray waxay leedahay daahirnimo sare iyo tayo wanaagsan, waxaana si ballaaran loogu isticmaali karaa PCR/qPCR-da hoose, NGS iyo cilmi-baaris kale oo tijaabo ah.
Astaamaha badeecada
◆ Ammaan ah oo aan sun ahayn: Looma baahna walxaha sunta ah ee dabiiciga ah sida phenol/chloroform
◆ Wax soo saar sare oo otomaatig ah: Iyada oo lagu qalabeeyay Bigfish Nucleic Acid Extractor, waxay samayn kartaa soo saarid sare waxayna ku habboon tahay soo saarista cabbirro muunad oo waaweyn
◆ Nadiifnimo sare iyo tayo wanaagsan: Badeecada la soo saaray waxay leedahay nadiifnimo sare waxaana loo isticmaali karaa NGS-ka hoose, isku-darka jajabka iyo tijaabooyin kale.
Habraacyada soo saarista
Saambal: Muunad cusub oo qiyaastii 100 mg ah ama muunad miisaan qalalan ah oo qiyaastii 30 mg ah
Shiidi: Si buuxda ugu shiidi nitrogen dareere ah ama mashiinka wax lagu shiido
Dheefshiidka: 65℃ dheefshiidka qubeyska diiran
Mashiinka: Ku shub meesha ka soo baxaysa oo ku dar saxanka si aad u soo saarto
Qalab la qabsan karo
Bigfish BFEX-32/BFEX-32E/BFEX-96E
Qeexitaanka badeecada
| Magaca Badeecada | Bisad. Maya. | Baakad |
| MagaSaafi ahOryza sativa L.Qalabka Nadiifinta DNA-da ee Genomic(pbaakad dib loo buuxiyay) | BFMP23R | 32T |
| MagaSaafi ahOryza sativa L.Qalabka Nadiifinta DNA-da ee Genomic (xirmo hore loo buuxiyay) | BFMP23R96 | 96T |
| Borotiinase K (p)urchase) | BFRD007 | 1ml/tuubo (10mg/ml) |
| RNase A(purchase) | BFRD017 | 1ml/tuubo (10mg/ml) |
Dejinta asturnaanta