Qalabka Sifaynta DNA Bakteeriyada MagPure

Sharaxaad Gaaban:

Ka dib marka lagu daaweeyo dareeraha dheefshiidka, asiidhka nucleic-ga ee ku jira muunadda bakteeriyada waxaa la sii daayaa oo keliya iyadoo la adeegsanayo Lysis Buffer. DNA-da genomic-ga ee la sii daayay waxay si gaar ah oo gaar ah ugu xidhan tahay kuullada Magnetic-ga. DNA-da genomic-ga ee ku xidhan walxaha birlabta waxaa qabta walxo birlabta ah; wasakhda waxaa laga saaraa iyadoo lagu dhaqo Wash Buffer. Asiidhka nucleic-ga ayaa markaa laga soo saaraa walxaha iyadoo la adeegsanayo Elution Buffer. Waxaa lagu dabaqi karaa dhammaan noocyada bakteeriyada garaam-negative iyo bakteeriyada togan, iwm.

NALA SOO XIRIIR

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Calaamadaha Alaabta

Qaybta ugu muhiimsan

Bisad.#	BFMP10R1	BFMP10R16	BFMP10R	Cqaybaha
Packing	1T40T/qalab	16T16T/qalab	32T32T/qalab
Borotiin K	400μ L x 2	320μ L	640μ L	Xalka borotiinka (Si gaar ah ayaa loo baakadeeyay)
RNaseA	80μ L	32μ L	64μ L	Xalka enzyme-ka (Si gaar ah ayaa loo baakadeeyay)
Kaydka BA	12 mL	5ml	10ml	Xalka milixda leh
Kaydka BL	Afartan iyo lix ceel diyaarinta. kaydadka 40 xabbo.	96 si fiican hore loo sii diyaariyey saxan 2 xabbo.	96 si fiican hore loo sii diyaariyey saxan 2 xabbo.	Denaturant xooggan iyo Tris baffer
Kaydka WA				Xalka milixda leh
Kaydka WB				Xal milix yar
Kaydka DE				Xalka Tris
Kubbado birlab ah				Xalka kuul birlab ah oo hydroxy ah
Buugga Isticmaalayaasha	1	1	1