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Qalabka Sifaynta DNA ee MagPure Plasmid
Hordhac kooban
Qalabkani waxa uu qaataa hab gaar ah oo la hagaajiyay oo la hagaajiyay iyo kuulo birlab ah oo si gaar ah ugu xidhan DNA-da, kuwaas oo si degdeg ah isku xidhi kara, u xajin kara, kala saari kara oo nadiifin kara asiidhyada nucleic-ka ah. Waxay aad ugu habboon tahay in si dhakhso ah oo hufan loo kala saaro loona nadiifiyo plasmid DNA laga bilaabo 0.5-2mL (caadiyan 1-1.5mL) dareeraha bakteeriyada, iyadoo meesha laga saarayo hadhaaga sida borotiinnada iyo ions milixda. Iyadoo la taageerayo isticmaalka Bigfish Magnetic Bead Nucleic Acid Extractor, waxay aad ugu habboon tahay soo saarista iswada ee cabbirrada muunada waaweyn. DNA-da la soo saaray ee plasmid waxay leedahay nadiif sare iyo tayo wanaagsan, waxaana si ballaaran loogu isticmaali karaa tijaabooyinka hoose sida dheefshiidka enzim, ligation, isbeddel, NGS, iwm..
Tilmaamaha Alaabta
Tayo wanaagsan:Go'doomi oo nadiifi plasmid DNA laga bilaabo 0.5-2mL xal bakteeriyada leh waxsoosaar sare iyo nadiif wanaagsan.
Degdeg ah oo fudud:Geedi socodka oo dhan uma baahna centrifugation soo noqnoqda ama hawlgallada sifaynta, taas oo ka dhigaysa mid ku habboon soo saarista cabbirrada muunada waaweyn.
Ammaan ah oo aan sun ahayn:Looma baahna reagents organic sun ah sida phenol/chloroform.
La qabsan karoQalabka
Bigfish: BFEX-32E, BFEX-32, BFEX-96E, BFEX-16E
Tilmaamaha alaabta
| AlaabtaNaamiin | Bisad Maya | Baakad |
| Qalabka Sifaynta DNA ee MagPure Plasmid (xirmo horay loo buuxiyay) | BFMP09R | 32T |
| Qalabka Sifaynta DNA ee MagPure Plasmid (xirmo horay loo buuxiyay) | BFMP09R1 | 40T |
| Qalabka Sifaynta DNA ee MagPure Plasmid (xirmo horay loo buuxiyay) | BFMP09R96 | 96T |
| RNaseA(iibso) | BFRD017 | 1ml/ tuubo(10mg/ml) |
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