SARS-Cov-2 Qalabka ogaanshaha Nucleic acid (flurescience rt-pcr)

Sharraxaadda Gaaban:

SARS-Cov-2 Qalabka ogaanshaha Nucleic acid (flurescicence rt-PCR) waxaa loo isticmaali karaa ogaanshaha aashitada nucciic ee 'coronavirus', oo ku habboon cudurka 'CDC', isbitaalada, xarunta imtixaanka ee xisbiga CDC-da iyo sheybaarka tacliinihiisa.

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Calaamadaha wax soo saarka

Astaamaha wax soo saarka

1, xasaasiyad sare: xadka ogaanshaha (lod)<2 × 102 nuqulo / ml.

2, TENTE TENTED HITE: Baaritaan hiddo-wadaha iyo n hiddo hal mar, u hoggaansanaanta ciladaha.

3, oo ku habboon qalab kala duwan: Abi 7500/755-kii; Roche feedhtcycler480; Biorad Cfx96; Garaadkeena ugu gaarka ah-bfqp96 / 48.

4, Soonka iyo Fudud: Reacent-ka horay loo qalay waa sahlan tahay in la isticmaalo, macaamiisha ayaa u baahan in lagu daro enzyme iyo shaashad. Qalabka soosaarka aashitada ee binifish ee Nucleic acid ayaa si fiican u isbarbar dhigaya tan. Adigoo adeegsanaya mashiinka soosaarka otomaatiga oo buuxa, waa dhakhso in laga shaqeeyo saamiyo badan.

5, Badbaadada Baabuur-nabdoon: waa dadka waaweyn eefish-ka waxay bixisaa muunad dareere ah oo loogu talagalay si dhakhso leh loogu talagalay fayraska si loo hubiyo badbaadada hawl wadeenada.